Aller au menu Aller au contenu
Physico chemistry of solids, thin films, biotechnologies
Applications for micro & nano- technologies, energy, health ...

> news

Thesis defence by Mirasbek KUTERBEKOV

Published on March 25, 2019
A+Augmenter la taille du texteA-Réduire la taille du texteImprimer le documentEnvoyer cette page par mail Partagez cet article Facebook Twitter Linked In Google+ Viadeo
PhD Defense May 6, 2019
Grenoble INP - Phelma
3 parvis Louis Néel - 38000 Grenoble
Accès : TRAM B arrêt Cité internationale
Free entrance - No registration
2:00 pm  room Z108 - Building Z - Phelma

Polymeric microcarriers with bioactive coatings for the osteogenic differentiation of human adipose stromal cells

mirasbek.jpg

mirasbek.jpg

Keywords :
microcarriers, human adipose stromal cells, polyelectrolyte coatings, bone morphogenetic proteins, osteogenic differentiation

click here to see the jury members


Abstract :
The regeneration of critical-sized bone defects remains a major healthcare challenge. The limitations of common tissue grafts prompted us to develop a synthetic alternative based on a biomaterial construct, osteoinductive factors and stem cells. For biomaterial construct, we focused on porous polymeric microcarriers as they support large-scale cell expansion and modular tissue assembly, circumventing two important bottlenecks for clinical translation. To insure industrial supply and regulatory approval, we designed an organic-solvent-free method for their fabrication based on the spherulitic crystallization of poly(L-lactide) (PLLA) in its blends with polyethylene glycol (PEG). The PLLA spherulites were easily recovered as microcarriers by rinsing away the water-soluble PEG. Their size and porosity could be independently controlled by tuning the PLLA/PEG ratio and crystallization temperature. The biocompatibility and osteoconductivity of PLLA microcarriers were confirmed through the expansion and osteogenic differentiation of human adipose stem cells (hASCs). Because the latter hASC function is sensitive to different culture parameters, we then used the Design of Experiments approach for their rapid screening. In combination with high-throughput analysis, we identified several parameters that had a pronounced influence on their osteogenic differentiation. Finally, for the delivery of osteoinductive factors, we elaborated polyelectrolyte multilayers (PEM) based on biocompatible poly(L-ornithine) and hyaluronic acid. These PEMs were characterized in terms of their growth, morphology, the ability to incorporate bone morphogenetic proteins (BMP) and to function as coatings on PLLA microcarriers. Our preliminary results showed that the incorporation of BMPs inside PEMs had a strong effect on hASC adhesion. While further studies are needed, hASC-seeded PLLA microcarriers coated with BMP-loaded PEMs could be a promising synthetic implant for improved bone regeneration.

jury members :
 

Prof.

Alain Jonas

IMCN, Université catholique de Louvain, Belgium

President

Directeur de Recherche

Philippe Lavalle

UMR 1121, INSERM, Université de Strasbourg, France

Rapporteur

Directeur de Recherche

Danièle Noël

IRMB, INSERM, Centre Hospitalier Régional Universitaire de Montpellier, France

Rapporteur

Prof.

Susanna Miettinen

BioMediTech, University of Tampere, Finland

Prof.

Karine Glinel

IMCN, Université catholique de Louvain, Belgium

Thesis Director

Prof.

Catherine Picart

LMGP, CNRS, Grenoble INP, France

Thesis Director


A+Augmenter la taille du texteA-Réduire la taille du texteImprimer le documentEnvoyer cette page par mail Partagez cet article Facebook Twitter Linked In Google+ Viadeo

Date of update March 25, 2019

Univ. Grenoble Alpes