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Physico chemistry of solids, thin films, biotechnologies
Applications for micro & nano- technologies, energy, health ...

> Research > IMBM

Bone tissue engineering

OBJECTIVES

We initially showed that bone morphogenetic proteins (BMPs) can be presented to cells in a matrix-bound manner, ie presented via a biomaterial surface (Crouzier et al, Small 2009) . This biomaterial is a thin film made by self-assembly, namely a layer-by-layer film of polysaccharides and polypeptides that has can trap the BMPs.
We have continued our development and proved, that BMP-coated ceramic granules are ostoinductive in an ectopic site, when they are implanted in muscle  (Guillot et al, Biomaterials 2011) . We further proved that BMP-2-loaded film-coated titanium implants can be dried and sterlized, while remaining osteoinductive (Guillot et al, Biomaterials 2013) . We then proved that a critical size femoral bone defect in rats can be regenerated by combining an initially empty 3D scaffold to the osteoinductive film coating. Notably, bone that was newly formed bone after 8 weeks exhibited the typical morphology of native bones, made of cortical and trabecular bones. Beside, the regenerated bone was vascularized (Bouyer et al, Biomaterials 2016).
In collaboration with Prof. Georges Bettega (CHR Annecy and IAB Grenoble), we are continuing our pre-clinical developments in large animals in the frame of the ERC POC REGENERBONE (PI CPicart)  and Fondation Gueules Cassées.
 

Researchers

Group Members


   Paul Machillot (Assistant Engineer)
   Michael Bouyer (PhD student)
   Charlotte Garot (PhD student)
   Sanela MORAND (M2 clinician)
 

Selected Publications

   

Surface delivery of tunable doses of BMP-2 from a polymeric scaffold induces volumetric bone regeneration

.
Bouyer M, Guillot R, Lavaud J, Plettinx C, Olivier C, Curry V, Boutonnat J, Coll JL, Peyrin F, Josserand V, Bettega G, Picart C. Surface delivery of tunable doses of BMP-2 from an adaptable polymeric scaffold induces volumetric bone regeneration. Biomaterials. 104:168-81, 2016. 

   

Assessment of a polyelectrolyte multilayer film coating loaded with BMP-2 on titanium and PEEK implants in the rabbit femoral condyle

 Guillot R, Pignot-Paintrand I, Lavaud J, Decambron A, Bourgeois E, Josserand V, Logeart-Avramoglou D, Viguier E, Picart CActa Biomaterialia 2016.

 
Spatio-temporal control of LbL films for biomedical applications: from 2D to 3D.
Monge C, Almodóvar J, Boudou T, Picart C.
Adv. Healthc. Mater. 2015.
Spatial patterning of BMP-2 and BMP-7 on biopolymer films and the guidance of muscle cell fate. 
Almodovar J, Guillot R, Monge C, Vollaire J, Seilimovic S, Coll JL,
Khademhosseini A, Picart C.
Biomaterials. 2014.
The stability of BMP loaded polyelectrolyte multilayer coatings on titanium.
Guillot R, Gilde F, Becquart P, Sailhan F, Lapeyrere A, Logeart-Avramoglou D, 
Picart C.
Biomaterials. 2013.
Secondary structure of rhBMP-2 in a protective biopolymeric carrier material.
Gilde F, Maniti O, Guillot R, Mano JF, Logeart-Avramoglou D, Sailhan F, Picart C.
Biomacromolecules. 2012.
The performance of BMP-2 loaded TCP/HAP porous ceramics with a polyelectrolyte multilayer film coating.
Crouzier T, Sailhan F, Becquart P, Guillot R, Logeart-Avramoglou D, Picart C.
Biomaterials. 2011.
Presentation of BMP-2 from a soft biopolymeric film unveils its activity on cell adhesion and migration.
Crouzier T, Fourel L, Boudou T, Albiges-Rizo C, Picart C. 
Adv. Mat. 2011.

Date of update October 9, 2019

Univ. Grenoble Alpes