Our initial studies (Crouzier et al, Small 2009) have shown that potent osteoinductive growth factors of the BMP family (Bone Morphogenetic Proteins) can be loaded in tunable amounts in layer-by-layer films made of biopolymers.
We have now taken two complementary routes: the first one consists in engineering new types of scaffolds for tissue engineering; the second aims at coating the surface of available impants with the biomimetic fimls contianing the BMPs. We have selected implants that are commonly used in orthopedic, dental and maxillo-facial surgery to enhance their osteoinductive properties. We showed that BMP-coated implants made of ceramics (Guillot et al, Biomaterials 2011) and of titanium (Guillot et al, Biomaterials 2013) are osteoinductive in a rat ectopic model. Recently, we proved that a critical size femoral bone defect in rats can be regenerated by combining an initially empty 3D scaffold to our osteoinductive coatings. Notably, the newly formed bone after 8 weeks exhibited typical properties of cortical as well as trabecular bone and was vascularized (Bouyer et al, Biomaterials 2016).