Actual in vitro models for skeletal muscle tissue engineering present many limitations for high content screening analysis: 2D models do not mimic the native cellular microenvironment, whereas 3D models require large amounts of cells, thus limiting the reproducibility of experiments. In our lab, we developed a microfabricated platform that enables the generation of arrays of 3D microtissues from low cell number while precisely controlling a large number of parameters, such as extracellular matrix composition, substrate rigidity, electrical or biochemical stimulations. Furthermore, this platform also allows to follow in real time microtissues contraction or calcium dynamics, as well as myoblast differentiation toward myotubes. Here, we demonstrate how tissue formation, stability, and cell differentiation in microtissues generated from immortalized human myoblasts can be improved by co-culturing the myoblasts with fibroblasts.
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