Aller au menu Aller au contenu
Synthèse et propriétés de monocristaux, de poudres, films minces ou hétérostructures

Etudes à l'interface avec la matière biologique

> LMGP_Présentation > LMGP_Actualites

LMGP Seminar : Dr. B. Le Pioufle - Micro/Nanotech for the analysis and the parallel treatment of cells or proteins on a chip

Publié le 9 janvier 2014
A+Augmenter la taille du texteA-Réduire la taille du texteImprimer le documentEnvoyer cette page par mail Partagez cet article Facebook Twitter Linked In Google+ Viadeo
Colloque / Séminaire 28 janvier 2014
Tuesday at 2:00pm - LMGP Conference room - 2nd floor
LMGP (UMR 5628 CNRS / Grenoble INP)
3 parvis Louis Néel - 38000 Grenoble

Accès : TRAM B arrêt Cité internationale

Free entrance - No registration 
Le Pioufle.jpg

Le Pioufle.jpg

B. Le Pioufle
SATIE-BIOMIS, CNRS, ENS de Cachan, 61 av du Pdt Wilson, 94230 Cachan, France
See his short biography

Micro/Nanotech for the analysis and the parallel treatment of cells or proteins on a chip


Abstract
One of the main potential of the micro and nanotechnologies for biological applications is the capability of such technologies to provide tools capable to handle, analyse or treat parallely a large number of biological compounds. This is due to the fact that those technologies, coming from the microelectronics, use collective fabrication means (many identical microsystems can be processed parallely on a same wafer).
Some examples of parallelized microdevices for the cell or cell components treatment or analysis will be screened during the presentation (DNA chips, Protein chips, cell chips). In particular the focus will be made of the biodevices for cell treatment.
The effects of electric field pulses on the cell membranes are now broadly used for transfection or drug insertion purposes (pulses having a typical amplitude of 1kV/cm and more than 1µs duration). Nevertheless the effects of ultra-short nanopulses (below 10ns duration, above 40kV/cm) on cell membranes and intracellular components (Beebe 2003, Napotnik 2012) are still under investigation within several research groups. In this context, our group is working toward the design and microfabrication of miniaturized biodevices devoted to the application of ultra short electrical pulses (nanopulses) to live cells, circulating within microfluidic channels or adherent in the exposition chamber. Main advantages of such are i) the miniaturization that allows the application of extremely high electrical fields (up 280 kV.cm-1 were achieved within our device) ii) real time observation and recording of the effects of nanopulses thanks to the transparency of the microfluidic device.
Microfluidic Device for adherent cells nanoporation
References
  • Beebe, S.J., White, J., Blackmore, P.F., Deng, Y.P., Somers, K., Schoenbach, K.H., 2003. DNA Cell Biol. 22 (12), 785–796
  • T. B. Napotnik, Y. H. Wu,, M. A. Gundersen, D. Miklavcˇicˇ and P. T. Vernier, Nanosecond electric pulses cause mitochondrial membrane permeabilization in Jurkat cells, Bioelectromagnetics, 2012, 33(3), 257–264.
  • C. Dalmay, J. Villemejane, V. Joubert, O. Francais, L. M. Mir and B. Le Pioufle Design and realization of a microfluidic device devoted to the application of ultra-short pulses of electrical field to living cells. Sensors and Actuators B: Chemical, 160, (1), 1573-1580, 2011
  • C. Dalmay, M. A. De Menorval, O. Francais, L. M. Mir and B. Le Pioufle A microfluidic device with removable packaging for the real time visualisation of intracellular effects of nanosecond electrical pulses on adherent cells. Lab on a Chip, 12, (22), 4709-15, 2012.
Short biography
Bruno Le Pioufle is Professor at Ecole Normale Supérieure de Cachan. He is responsible of the research group BIOMIS (Biomicrosystems). His main scientific concerns are 1) the conception of microfluidic biochips for the monitoring of membrane proteins and ion channels and 2) the conception of microfluidic biochips for the cell handling and treatment.
He spent two years from 2005 at University of Tokyo, where is was at the Direction of an international laboratory between CNRS and the University of Tokyo (Laboratory of Integrated Micro Mechatronics Systems, UMI CNRS 2820).
He founded the group BIOMIS in 2002 (currently 15 members) belonging to UMR CNRS 8029, and Institut Fédératif de Recherche CNRS FR3242.
He has been responsible of several international or local research projects (JST-CNRS, ANR, Labex projects, CNANO ile de France, CNRS).

  • Bisceglia, E., Cubizolles, M., Mallard, F., Vinet, F., Français, O., & Le Pioufle, B. (2013). Micro-organism extraction from biological samples using DEP forces enhanced by osmotic shock. Lab on a chip. doi:10.1039/c2lc41128h
  • Dalmay, C., De Menorval, M. A., Français, O., Mir, L. M., & Le Pioufle, B. (2012). A microfluidic device with removable packaging for the real time visualisation of intracellular effects of nanosecond electrical pulses on adherent cells. Lab on a chip, 12(22), 4709–15. doi:10.1039/c2lc40857k
  • Mottet, B. Le Pioufle and L. M. Mir (2012). High-resolution analyses of cell fusion dynamics in a biochip. ELECTROPHORESIS, 33, (16), 2508-2515,
  • B. Hajj, S. De Reguardati, L. Hugonin, B. Le Pioufle, T. Osaki, H. Suzuki, S. Takeuchi, H. Mojzisova, D. Chauvat and J. Zyss (2009). Electro-Optical Imaging Microscopy of Dye-Doped Artificial Lipidic Membranes. Biophysical Journal, 97, (11), 2913-2921,
  • B. Le Pioufle, H. Suzuki, K. Tabata, H. Noji and S. Takeuchi , (2008). Lipid Bilayer Microarray for Parallel Recording of Transmembrane Ion Currents. Analytical chemistry, 80, (1), 328-332

A+Augmenter la taille du texteA-Réduire la taille du texteImprimer le documentEnvoyer cette page par mail Partagez cet article Facebook Twitter Linked In Google+ Viadeo

mise à jour le 22 janvier 2015

  • Tutelle CNRS
  • Tutelle Grenoble INP
Univ. Grenoble Alpes