We develop 2D platforms able to mimic defined aspects of the extracellular matrix, for molecular and cellular studies.Surfaces functionalization with glycans, adhesion peptides, growth factors and chemokines is precisely controlled in terms of molecular surface density and orientation. These well-defined platforms can be used for cellular studies: differentiation, migration, signaling, and molecular cross-talks (more info).
THERAPEUTIC PROTEIN AGGREGATION AT MATERIAL SURFACES
We have developed sensitive biochemical and biophysical techniques to study protein adsorption and aggregation kinetics in a quantitative and qualitative way on the material surface. Our model protein is insulin and its material-induced aggregation into amyloid fibers. More recently, we are also analyzing the effect of dynamic air-liquid interfaces on protein aggregation. (more info).
PROTEIN INTERACTIONS WITH BIOMIMETIC MEMBRANES
Ezrin and Moesin, members of the ezrin/radixin/moesin (ERM) family, are membrane/cytoskeleton linkers involved in cell morphogenesis and motility. The aim of this project is to understand at the molecular level the link between membrane structures and the actin cytoskeleton. To this end, we produce recombinant ERM proteins and engineer biomimetic lipid membranes. (more info).