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New IMBM paper by Karim

Published on September 17, 2015
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News, Communique August 11, 2015

Congratulations Karim Chouchane! His new paper on the aggregation of human insulin is published in J. Phys. Chem. B!

Chouchane_2015.jpeg

Chouchane_2015.jpeg

Here is Karim's full paper, and here is the abstract:
Soluble proteins are constantly in contact with material or cellular surfaces, which can trigger their aggregation and therefore have a serious impact on the development of stable therapeutic proteins. In contact with hydrophobic material surfaces, human insulin aggregates readily into amyloid fibers. The kinetics of this aggregation can be accelerated by small peptides, forming stable beta-sheets on hydrophobic surfaces. Using a series of (LK)nL peptides with varying length, we show that these peptides, at low, substoichiometric concentrations, have a positive, cooperative effect on insulin aggregation. This effect is based on a cooperative adsorption of (LK)nL peptides at hydrophobic surfaces, where they form complexes that help the formation of aggregation nuclei. At higher concentrations, they interfere with the formation of an aggregative nucleus. These effects are strictly dependent on the their adsorption on hydrophobic material surfaces and highlight the importance of the impact of materials on protein stability. (LK)nL peptides prove to be valuable tools to investigate the mechanism of HI aggregation nuclei formation on hydrophobic surfaces.
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Written by Thomas Boudou

Date of update January 7, 2017

Communauté Université Grenoble Alpes