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Grenoble INP
Physico chemistry of solids, thin films, biotechnologies
Applications for micro & nano- technologies, energy, health ...

Protein interactions with biomimetic lipid membranes

OBJECTIVES

The aim of this project is to understand at the molecular level the link between membrane structures and the actin cytoskeleton. Ezrin and Moesin, members of the ezrin/radixin/moesin (ERM) family, are membrane/cytoskeleton linkers involved in cell morphogenesis and motility.
Our goal is to study ERM proteins/lipid membrane/cytoskeleton interactions in vitro in biomimetic systems in order to better understand the underlying molecular processes. To this end, we produce recombinant ERM proteins and mutants and engineer biomimetic lipid membranes, especially LUVs (large unilamellar vesicles) and supported lipid bilayers (SLBs). Molecular interactions are studied by a large set of biophysical techniques (QCM-D, AFM, FTIR spectroscopy, fluorescence spectroscopy, and TIRF).

Researchers

Group Members

   Quentin Lubart (PhD student)
   Fabien Dalonneau (Assistant engineer)

Selected Publications

Binding of moesin and ezrin to membranes containing phosphatidylinositol(4,5) bisphosphate: a comparative study of the affinity constants and conformational changes. 
Maniti O, Kalifa N, Goggia L, Dalonneau F, Guérin C, Blanchoin L, Ramos L, Picart C.
Biochim Biophys. Acta. 2012. 
Targeting of MuLV Gag to the plasma membrane is mediated by PI(4,5)P2/PS and a polybasic region in the Matrix
Hamard-Peron E, Juilliard F, Saad JS, Roy C, Roingeard P, Summers MF, Darlix JL, Picart C, Muriaux D.
J. Virol. 84. 2010.

Written by Colette Lartigue

Date of update March 11, 2015

Communauté Université Grenoble Alpes
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