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Physico chemistry of solids, thin films, biotechnologies
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Publication by Valerie Stambouli

Published on January 26, 2017
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Communique from December 6, 2016 to December 13, 2016

The paper "Surface–Enhanced Raman Spectroscopy Label–free Detection of DNA Complementary PolyA and PolyT Polybases on Ag/TiO2 Platform" has been published in ChemistrySelect

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Here  you will find the paper by Valérie Stambouli

"An original, robust, and low cost Ag°/TiO2 platform has been used for the Surface-Enhanced Raman Spectroscopy (SERS) detection of polyA-NH2 and polyT complementary bases. Three procedures have been assessed to immobilize the polybases in various concentrations on the SERS platform, namely immobilization of polybases alone, immobilization of the polyA-NH2+polyT mixture, and two-step immobilization of the polyA-NH2.polyT system. Derived SERS spectra have been analyzed and are discussed in terms of spectrum reproducibility, influence of polybase concentration in solution, orientation of polybases at the SERS platform surface, as well as feasibility of complementary polybase hybridization. This study allows us envisaging a simple hybridization procedure using the polyA-NH2 .polyT system and an efficient SERS detection of in situ hybridization features through intensity measurements of the
adenine vibration mode at 1330 cm-1."


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Written by Maria Carmen Jimenez Arevalo

Date of update January 26, 2017

Communauté Université Grenoble Alpes